Human FGFR1 (Fibroblast Growth Factor Receptor 1) ELISA Kit
The Human FGFR1 (Fibroblast Growth Factor Receptor 1) ELISA Kit is specifically designed for the precise measurement of FGFR1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.FGFR1 is a key receptor involved in the signaling pathways related to cell growth, differentiation, and development.
Dysregulation of FGFR1 has been linked to various diseases, including cancer, skeletal disorders, and developmental abnormalities. Therefore, this ELISA kit serves as a valuable tool in understanding the roles of FGFR1 in disease pathogenesis and potential therapeutic interventions.Visit the provided URL for more detailed information on the Human FGFR1 ELISA Kit from AssayGenie.
Product Name:
Human FGFR1 (Fibroblast Growth Factor Receptor 1) ELISA Kit
SKU:
HUES01564
Target:
Human FGFR1 (Fibroblast Growth Factor Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGFR1. You can calculate the concentration of Human FGFR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
98-113
89-103
Average (%)
93
105
95
1:4
Range (%)
86-101
84-97
87-99
Average (%)
93
89
93
1:8
Range (%)
91-103
80-94
82-92
Average (%)
97
86
87
1:16
Range (%)
95-106
79-90
89-102
Average (%)
100
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.85
4.1
0.46
0.88
4.02
Standard deviation
0.03
0.04
0.16
0.03
0.05
0.14
C V (%)
6.12
4.71
3.9
6.52
5.68
3.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGFR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR1 in samples. No significant cross-reactivity or interference between Human FGFR1 and analogues was observed.
