The Human FG Alpha (Fibrinogen Alpha) CLIA Kit is a reliable and accurate tool for measuring levels of FG Alpha in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.FG Alpha, a key component of fibrinogen, is essential for proper blood clotting and wound healing.
Dysregulation of FG Alpha levels has been linked to various inflammatory and cardiovascular conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Enhance your research with the Human FG Alpha CLIA Kit and gain valuable insights into the role of FG Alpha in health and disease. Visit the provided URL for more information and to order your kit today!
Product Name:
Human FG alpha (Fibrinogen Alpha) CLIA Kit
SKU:
HUES01126
Target:
Human FG alpha (Fibrinogen Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGα. You can calculate the concentration of Human FGα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
85-101
102-114
Average (%)
102
92
107
1:4
Range (%)
97-112
101-115
94-107
Average (%)
104
109
101
1:8
Range (%)
91-101
93-105
88-104
Average (%)
96
99
95
1:16
Range (%)
98-114
91-106
92-106
Average (%)
106
98
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
94-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.49
11.14
43.66
5.94
11.36
47.43
Standard deviation
0.47
1.31
3.97
0.73
1.24
3.25
C V (%)
8.56
11.76
9.09
12.29
10.92
6.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGα in samples. No significant cross-reactivity or interference between Human FGα and analogues was observed.
