The Human FETUB (Fetuin B) ELISA Kit is designed to quantitatively detect Fetuin B levels in human biological samples. Fetuin B, a member of the cystatin superfamily of cysteine protease inhibitors, plays crucial roles in various cellular processes, including protease regulation and cell differentiation. Its measurement provides valuable insights into specific physiological functions and potential implications in diverse biological pathways. Our FETUB ELISA Kit assures exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research or clinical investigations.
Manufactured under strict quality control measures, this kit delivers reliable performance and user-friendly protocols for seamless application. Rely on Assay Genie's FETUB ELISA Kit for the precise quantification of this biomarker, facilitating a deeper understanding of its biological significance.
Product Name:
Human FETUB (Fetuin B) ELISA Kit
SKU:
AEES00131
Target:
Human FETUB (Fetuin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FETUB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FETUB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FETUB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FETUB. You can calculate the concentration of Human FETUB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
92-108
93-101
Average (%)
94
100
96
1:4
Range (%)
91-103
94-105
86-97
Average (%)
97
100
90
1:8
Range (%)
93-105
86-96
99-110
Average (%)
97
93
105
1:16
Range (%)
97-107
88-100
94-104
Average (%)
102
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-110
103
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
92-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.85
2.34
9.35
0.84
2.35
8.73
Standard deviation
0.05
0.11
0.38
0.04
0.12
0.43
C V (%)
5.88
4.7
4.06
4.76
5.11
4.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FETUB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FETUB in samples. No significant cross-reactivity or interference between Human FETUB and analogues was observed.
