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Human FEN1 Recombinant Protein (RPPB1637)

SKU:
RPPB1637
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P39748
Research Area:
Enzymes
€309
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Description

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Product Name:Human FEN1 Recombinant Protein
Product Code:RPPB1637
Size:20µg
Species:Human
Target:FEN1
Synonyms:FEN-1, MF1, RAD2, Maturation Factor-1, MF-1, Flap endonuclease 1, Flap structure-specific endonuclease 1, Maturation factor 1, hFEN-1, DNase IV, FEN1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The protein contains 20mM Tris-HCl buffer pH-8.0, 1mM DTT, 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

FEN1 removes 5'' overhanging flaps in DNA repair and processes the 5'' ends of Okazaki fragments in lagging strand DNA synthesis. The interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, therefore passing the substrate from one enzyme to another. FEN1 is part of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5'' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

FEN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-380 a.a.) and having a molecular mass of 42.5 kDa. The FEN1 protein is purified by standard chromatogrpahy techniques.

UniProt Protein Function:FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
UniProt Protein Details:

Protein type:Nucleolus; EC 3.1.-.-; Nuclear receptor co-regulator; DNA repair, damage; Ribonuclease; DNA-binding; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: membrane; mitochondrion; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; nucleus; plasma membrane; protein complex

Molecular Function:5'-3' exonuclease activity; 5'-flap endonuclease activity; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded DNA specific exodeoxyribonuclease activity; endonuclease activity; exonuclease activity; protein binding; ribonuclease H activity

Biological Process: DNA repair; DNA replication; DNA replication, removal of RNA primer; double-strand break repair; double-strand break repair via homologous recombination; telomere maintenance via recombination; UV protection

NCBI Summary:The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
UniProt Code:P39748
NCBI GenInfo Identifier:729475
NCBI Gene ID:2237
NCBI Accession:P39748.1
UniProt Related Accession:P39748,AAB32522
Molecular Weight:35,673 Da
NCBI Full Name:Flap endonuclease 1
NCBI Synonym Full Names:flap structure-specific endonuclease 1
NCBI Official Symbol:FEN1��
NCBI Official Synonym Symbols:MF1; RAD2; FEN-1��
NCBI Protein Information:flap endonuclease 1
UniProt Protein Name:Flap endonuclease 1
UniProt Synonym Protein Names:DNase IV; Flap structure-specific endonuclease 1
UniProt Gene Name:FEN1��
UniProt Entry Name:FEN1_HUMAN

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