Human FDP (Fibrinogen Degradation Product) CLIA Kit
The Human FDP (Fibrinogen Degradation Product) CLIA Kit is specifically designed for the quantitative detection of FDP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Fibrinogen degradation products are important markers for monitoring coagulation and fibrinolysis processes in conditions such as disseminated intravascular coagulation, thrombosis, and liver diseases.
By measuring FDP levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify novel therapeutic strategies.The Human FDP CLIA Kit from Assay Genie provides researchers with a reliable and efficient tool for studying coagulation disorders and evaluating patient responses to treatment. With its high-performance characteristics, this kit is a valuable asset for laboratories conducting research in the field of hemostasis and thrombosis.
Product Name:
Human FDP (Fibrinogen Degradation Product) CLIA Kit
SKU:
HUES01156
Target:
Human FDP (Fibrinogen Degradation Product)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FDP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FDP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FDP. You can calculate the concentration of Human FDP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
93-104
84-98
Average (%)
91
98
90
1:4
Range (%)
90-103
102-118
86-96
Average (%)
96
109
91
1:8
Range (%)
90-104
103-119
96-111
Average (%)
96
109
104
1:16
Range (%)
88-100
86-100
86-101
Average (%)
95
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.86
5.54
14.18
1.69
6.07
15.58
Standard deviation
0.17
0.66
0.96
0.18
0.56
1.38
C V (%)
9.14
11.91
6.77
10.65
9.23
8.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FDP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FDP in samples. No significant cross-reactivity or interference between Human FDP and analogues was observed.
