Human FcgammaR3B (Fc Fragment of IgG Low Affinity IIIb Receptor) CLIA Kit
The Human Fc Gamma RIIIb (FCG3B) CLIA Kit is a cutting-edge assay designed for the precise detection of FCG3B levels in human serum, plasma, and cell culture supernatants. This kit provides unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.FCG3B is a pivotal receptor involved in the immune response, specifically in antibody-dependent cellular cytotoxicity (ADCC) and immune complex clearance. Dysregulation of FCG3B has been linked to various autoimmune diseases, infectious diseases, and cancer, making it a crucial biomarker for studying disease mechanisms and developing targeted therapies.
The Human FCG3B CLIA Kit from Assay Genie is a valuable tool for researchers and clinicians seeking to deepen their understanding of immune responses and identify potential therapeutic targets. With its reliability and precision, this kit is an essential addition to any laboratory conducting immunological studies.
Product Name:
Human FcgammaR3B (Fc Fragment of IgG Low Affinity IIIb Receptor) CLIA Kit
SKU:
HUES00970
Target:
Human FcgammaR3B (Fc Fragment of IgG Low Affinity IIIb Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FcγR3B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγR3B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγR3B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FcγR3B. You can calculate the concentration of Human FcγR3B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
92-108
86-100
Average (%)
97
99
91
1:4
Range (%)
84-100
87-101
96-113
Average (%)
91
94
104
1:8
Range (%)
89-102
86-100
96-113
Average (%)
95
93
104
1:16
Range (%)
91-103
96-110
102-117
Average (%)
96
101
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
99-115
105
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.76
524.79
1772.04
186.97
504.82
1603.83
Standard deviation
23.57
56.36
117.31
17.03
57.6
112.43
C V (%)
11.51
10.74
6.62
9.11
11.41
7.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FcγR3B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FcγR3B in samples. No significant cross-reactivity or interference between Human FcγR3B and analogues was observed.
