The Human FcGamma (Fc Fragment of IgG) ELISA Kit is specially designed for the precise measurement of Fc fragment of IgG levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The Fc fragment of IgG plays a crucial role in immune responses, mediating antibody-dependent cellular cytotoxicity and other immune functions.
Abnormalities in FcGamma levels are associated with autoimmune diseases, inflammatory disorders, and immune deficiencies, making it a valuable biomarker for studying these conditions and developing potential treatments.With its reliable performance and broad applicability, the Human FcGamma ELISA Kit is an indispensable tool for researchers seeking to investigate the role of Fc fragment of IgG in health and disease.
Product Name:
Human Fcgamma (Fc Fragment of IgG) ELISA Kit
SKU:
HUES02725
Target:
Human Fcgamma (Fc Fragment of IgG)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Fcγ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Fcγ. You can calculate the concentration of Human Fcγ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
83-97
96-109
Average (%)
91
90
101
1:4
Range (%)
88-99
80-92
84-96
Average (%)
93
86
90
1:8
Range (%)
87-98
81-92
82-94
Average (%)
92
86
88
1:16
Range (%)
88-101
85-98
88-101
Average (%)
94
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
1.7
7.38
1.09
1.81
7.69
Standard deviation
0.06
0.08
0.3
0.07
0.09
0.38
C V (%)
5.94
4.71
4.07
6.42
4.97
4.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Fcγ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Fcγ in samples. No significant cross-reactivity or interference between Human Fcγ and analogues was observed.
