The Human FBLN4 (Fibulin-4) CLIA Kit is specifically formulated for the accurate measurement of Fibulin-4 levels in human samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.Fibulin-4 is a key protein that plays a vital role in regulating elastic fiber formation and tissue homeostasis. Dysregulation of Fibulin-4 has been implicated in various diseases such as cardiovascular disorders and connective tissue diseases, underscoring its importance as a biomarker for studying these conditions and exploring potential treatment options.
By utilizing the Human FBLN4 CLIA Kit, researchers can gain valuable insights into the role of Fibulin-4 in disease pathogenesis and progression, ultimately paving the way for the development of targeted therapies and personalized medicine approaches.
Product Name:
Human FBLN4 (Fibulin 4) CLIA Kit
SKU:
HUES00270
Target:
Human FBLN4 (Fibulin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FBLN4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBLN4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FBLN4. You can calculate the concentration of Human FBLN4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
99-114
99-111
Average (%)
101
107
105
1:4
Range (%)
98-113
88-101
97-109
Average (%)
105
93
103
1:8
Range (%)
98-111
96-113
96-109
Average (%)
105
103
103
1:16
Range (%)
86-97
88-100
97-112
Average (%)
91
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.85
5.34
16.51
2.03
5.72
16.91
Standard deviation
0.19
0.62
1.45
0.24
0.47
1.94
C V (%)
10.27
11.61
8.78
11.82
8.22
11.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FBLN4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBLN4 in samples. No significant cross-reactivity or interference between Human FBLN4 and analogues was observed.
