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Human FBL Recombinant Protein (RPPB1632)

SKU:
RPPB1632
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22087
Research Area:
Enzymes
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Description

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Product Name:Human FBL Recombinant Protein
Product Code:RPPB1632
Size:20µg
Species:Human
Target:FBL
Synonyms:rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, FBL, FIB1, FLRN, fibrillarin, FIB, RNU3IP1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The FBL solution contains Phosphate buffered saline (pH 7.4), 30% glycerol, and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN

FBL is a significant small nucleolar protein in eukaryotes, which has an essential role in pre-rRNA processing during ribosomal biogenesis. Fibrillarin is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Fibrillarin contains an N-terminal repetitive domain which is rich in glycine and arginine residues, like fibrillarins in other species. Fibrillarin�s central region is similar to an RNA-binding domain and contains an RNP consensus sequence. FBL is linked to the U3, U8, and U13 small nuclear RNAs and is positioned in the dense fibrillar component (DFC) of the nucleolus. Antisera from roughly 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

FBL Human Recombinant fused with 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 262 amino acids (83-321 a.a.) and having a molecular mass of 28.9kDa. The FBL is purified by proprietary chromatographic techniques.

UniProt Protein Function:FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.
UniProt Protein Details:

Protein type:EC 2.1.1.-; RNA-binding; Nucleolus; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function:protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing

NCBI Summary:This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
UniProt Code:P22087
NCBI GenInfo Identifier:14549159
NCBI Gene ID:2091
NCBI Accession:P22087.2
UniProt Secondary Accession:P22087,O75259, Q6IAT5, Q9UPI6, B5BUE8,
UniProt Related Accession:P22087
Molecular Weight:321
NCBI Full Name:rRNA 2'-O-methyltransferase fibrillarin
NCBI Synonym Full Names:fibrillarin
NCBI Official Symbol:FBL��
NCBI Official Synonym Symbols:FIB; FLRN; RNU3IP1��
NCBI Protein Information:rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; histone-glutamine methyltransferase; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
UniProt Protein Name:rRNA 2'-O-methyltransferase fibrillarin
UniProt Synonym Protein Names:34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase
Protein Family:rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name:FBL��
UniProt Entry Name:FBRL_HUMAN

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