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Human Factor Related Apoptosis (FAS) ELISA Kit

SKU:
HUDL01020
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25445
Sensitivity:
12pg/mL
Range:
39.062-2500pg/mL
ELISA Type:
Sandwich
Synonyms:
CD95,ALPS1A,ALPS1-A,APO1,APT1,FAS1,FASTM,TNFRSF6,Fas Receptor,TNF Receptor Superfamily Member 6,Tumor Necrosis Factor Receptor Superfamily Member 6
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Factor Related Apoptosis (FAS) ELISA Kit

The Human Factor-Related Apoptosis Fas ELISA Kit is a cutting-edge tool for researchers looking to accurately measure Fas levels in human samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.Fas, also known as CD95, is a cell surface receptor that plays a key role in programmed cell death, or apoptosis. Dysregulation of Fas signaling has been implicated in a range of diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.

By accurately measuring Fas levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets.With its user-friendly protocol and robust performance, the Human Factor-Related Apoptosis Fas ELISA Kit is an essential tool for any laboratory studying apoptosis and cell death pathways. Don't miss out on the opportunity to elevate your research with this top-of-the-line ELISA kit.

Product Name:Human Factor Related Apoptosis (FAS) ELISA Kit
Product Code:HUDL01020
Size:96 Assays
Target:FAS
Synonyms:CD95, ALPS1A, ALPS1-A, APO1, APT1, FAS1, FASTM, TNFRSF6, Fas Receptor, TNF Receptor Superfamily Member 6, Tumor Necrosis Factor Receptor Superfamily Member 6
Detection Method:Sandwich
Range:39.062-2500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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