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Human Fatty Acid Binding Protein 7, Brain (FABP7) ELISA Kit

SKU:
HUDL01008
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15540
Sensitivity:
0.18ng/mL
Range:
0.468-30ng/mL
ELISA Type:
Sandwich
Synonyms:
B-FABP,BLBP,FABPB,MRG,Brain Lipid Binding Protein,Mammary-derived growth inhibitor related
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Fatty Acid Binding Protein 7, Brain (FABP7) ELISA Kit

The Human Fatty Acid Binding Protein 7 (Brain FABP7) ELISA Kit is a powerful tool for accurately measuring levels of FABP7 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it perfect for a variety of research applications.FABP7, also known as Brain Fatty Acid Binding Protein, is a key protein involved in regulating fatty acid transport and metabolism in the brain.

Its expression has been linked to various neurological conditions including Alzheimer's disease, stroke, and brain tumors, highlighting its importance as a biomarker for studying these disorders and developing potential treatments.By using the Human FABP7 ELISA Kit, researchers can gain valuable insights into the role of FABP7 in brain health and disease, paving the way for advancements in neuroscience and personalized medicine.

Product Name:Human Fatty Acid Binding Protein 7, Brain (FABP7) ELISA Kit
Product Code:HUDL01008
Size:96 Assays
Target:FABP7
Synonyms:B-FABP, BLBP, FABPB, MRG, Brain Lipid Binding Protein, Mammary-derived growth inhibitor related
Detection Method:Sandwich
Range:0.468-30ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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