Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
The Human FABP4 (Fatty Acid Binding Protein 4) ELISA Kit is designed for the accurate detection of FABP4 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.FABP4 is a key protein involved in fatty acid metabolism and adipocyte function. Its levels have been implicated in various metabolic disorders such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
Overall, the Human FABP4 ELISA Kit provides researchers with a powerful tool for studying the role of FABP4 in metabolic and cardiovascular diseases, allowing for precise and accurate measurements of FABP4 levels in biological samples.
Product Name:
Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
SKU:
HUES01520
Target:
Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FABP4. You can calculate the concentration of Human FABP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
93-109
89-101
Average (%)
98
101
95
1:4
Range (%)
91-105
84-96
87-98
Average (%)
97
90
93
1:8
Range (%)
87-99
83-93
87-100
Average (%)
93
88
93
1:16
Range (%)
91-104
83-95
85-96
Average (%)
97
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.23
2.72
9.72
1.2
2.74
9.02
Standard deviation
0.07
0.13
0.39
0.07
0.12
0.32
C V (%)
5.69
4.78
4.01
5.83
4.38
3.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FABP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed.
