Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
The Human FABP3 (Fatty Acid Binding Protein 3) Muscle and Heart ELISA Kit is designed for the precise measurement of FABP3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.FABP3 is a key protein involved in fatty acid metabolism and is predominantly found in muscle and heart tissues.
Its levels can indicate muscle and heart damage, making it a valuable biomarker for studying conditions such as heart attacks, heart failure, and skeletal muscle diseases. This ELISA kit is an essential tool for researchers looking to investigate these conditions and develop potential treatments.
Product Name:
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
SKU:
HUES02467
Target:
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FABP3. You can calculate the concentration of Human FABP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
95-108
86-98
Average (%)
99
102
91
1:4
Range (%)
94-107
81-94
82-97
Average (%)
100
88
88
1:8
Range (%)
87-100
83-95
89-102
Average (%)
93
90
94
1:16
Range (%)
89-103
86-102
83-96
Average (%)
96
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.17
3.73
0.52
1.12
3.53
Standard deviation
0.03
0.07
0.15
0.03
0.05
0.13
C V (%)
5.45
5.98
4.02
5.77
4.46
3.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FABP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP3 in samples. No significant cross-reactivity or interference between Human FABP3 and analogues was observed.
