Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
The Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit is meticulously crafted to enable the quantitative detection of FABP1 levels in various biological samples of human origin. Fatty Acid Binding Protein 1, primarily expressed in the liver, is involved in intracellular fatty acid transport and metabolism, playing a crucial role in lipid homeostasis and metabolic processes. Accurate measurement of FABP1 can provide insights into lipid metabolism, liver function, and metabolic disorders, making it a valuable tool for research in liver-related pathologies.
Our ELISA kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results essential for studying FABP1's function and diagnostic potential. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for cutting-edge research applications. Trust Assay Genie's FABP1 ELISA Kit for accurate and dependable quantification of this vital biomarker in your investigations.
Product Name:
Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
SKU:
AEES00161
Target:
Human FABP1 (Fatty Acid Binding Protein 1, Liver)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FABP1. You can calculate the concentration of Human FABP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
84-98
95-112
Average (%)
91
91
102
1:4
Range (%)
86-102
86-98
97-113
Average (%)
93
91
104
1:8
Range (%)
86-99
83-96
96-109
Average (%)
91
88
103
1:16
Range (%)
86-101
86-98
95-107
Average (%)
93
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.41
3.75
13.67
1.47
3.66
14.82
Standard deviation
0.08
0.15
0.62
0.1
0.16
0.54
C V (%)
5.67
4.05
4.56
6.87
4.26
3.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FABP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP1 in samples. No significant cross-reactivity or interference between Human FABP1 and analogues was observed.
