The Human Coagulation Factor VIII (F8) ELISA Kit is a specialized assay meticulously developed to enable the precise quantitative measurement of Factor VIII levels in human biological samples. Factor VIII is a critical coagulation protein that plays a key role in the blood clotting cascade, contributing to hemostasis and clot formation. Dysregulation or deficiency of Factor VIII can lead to bleeding disorders, making accurate quantification essential for evaluating coagulation status and monitoring treatment responses.
Our F8 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in research settings. Manufactured under strict quality control measures, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for investigating Factor VIII levels in various research applications. Trust Assay Genie for dependable quantification and insightful analysis of Factor VIII biomarkers in your studies.
Product Name:
Human F8 (Coagulation Factor Ⅷ) ELISA Kit
SKU:
AEES00146
Target:
Human F8 (Coagulation Factor Ⅷ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 IU/mL
Detection range:
0.16-10 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F8. You can calculate the concentration of Human F8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
95-108
88-99
Average (%)
93
100
96
1:4
Range (%)
91-101
100-110
87-97
Average (%)
95
105
90
1:8
Range (%)
84-99
98-112
83-97
Average (%)
92
104
90
1:16
Range (%)
93-108
92-100
88-97
Average (%)
100
96
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-109
102
EDTA plasma (n=5)
87-96
90
Cell culture media (n=5)
96-108
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.44
1.19
4.01
0.49
1.02
4.03
Standard deviation
0.02
0.06
0.17
0.03
0.05
0.21
C V (%)
4.55
5.04
4.24
6.12
4.9
5.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
