The Human F12 (Coagulation Factor XII) CLIA Kit is specifically designed for the quantitative determination of Coagulation Factor XII levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and reproducible results, making it a valuable tool for researchers studying coagulation disorders, thrombosis, and other related conditions.Coagulation Factor XII is a critical factor in the blood clotting cascade, playing a key role in the initiation of the intrinsic pathway.
Dysregulation of Factor XII has been implicated in various thrombotic disorders, making it an important biomarker for understanding and diagnosing these conditions.With this CLIA kit, researchers can confidently measure Coagulation Factor XII levels in clinical samples, enabling a better understanding of coagulation processes and facilitating the development of novel diagnostic and therapeutic strategies for coagulation-related disorders.
Product Name:
Human F12 (Coagulation Factor XII) CLIA Kit
SKU:
HUES00479
Target:
Human F12 (Coagulation Factor XII)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F12. You can calculate the concentration of Human F12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
98-112
93-106
Average (%)
96
104
101
1:4
Range (%)
83-96
88-100
95-112
Average (%)
90
93
103
1:8
Range (%)
101-118
97-111
100-113
Average (%)
109
104
106
1:16
Range (%)
91-104
101-118
99-115
Average (%)
98
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.84
103.28
476.02
52.37
104.88
506.95
Standard deviation
5.38
7.28
36.03
4.66
10.8
30.67
C V (%)
9.99
7.05
7.57
8.9
10.3
6.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F12 in samples. No significant cross-reactivity or interference between Human F12 and analogues was observed.
