The Human EXT1 (Exostoses 1) CLIA Kit is a reliable and accurate assay designed for the detection of EXT1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing consistent and reproducible results for a variety of research applications.EXT1, also known as Exostoses 1, is a critical protein involved in the regulation of heparan sulfate synthesis and cell signaling pathways. Mutations in the EXT1 gene have been linked to hereditary multiple exostoses, a rare genetic disorder characterized by the formation of multiple cartilage-capped tumors called exostoses.
The Human EXT1 CLIA Kit can aid in the investigation of the role of EXT1 in disease development and progression, as well as in the development of potential therapeutic interventions.Overall, the Human EXT1 CLIA Kit is a valuable tool for researchers studying EXT1-related disorders and seeking to better understand the molecular mechanisms involved in these conditions. Its high sensitivity and specificity make it an ideal choice for precise and reliable measurements of EXT1 levels in human samples.
Product Name:
Human EXT1 (Exostoses 1) CLIA Kit
SKU:
HUES00800
Target:
Human EXT1 (Exostoses 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EXT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EXT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EXT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EXT1. You can calculate the concentration of Human EXT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
100-115
94-108
Average (%)
109
106
100
1:4
Range (%)
91-102
94-106
92-106
Average (%)
96
101
99
1:8
Range (%)
88-104
84-96
92-105
Average (%)
95
91
99
1:16
Range (%)
96-107
95-107
86-102
Average (%)
102
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
104
EDTA plasma (n=5)
96-106
101
Cell culture media (n=5)
96-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.42
4.28
0.49
1.38
4.1
Standard deviation
0.06
0.14
0.44
0.05
0.1
0.47
C V (%)
11.32
9.86
10.28
10.2
7.25
11.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EXT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EXT1 in samples. No significant cross-reactivity or interference between Human EXT1 and analogues was observed.
