The Human Endothelin Receptor A CLIA Kit is specifically designed for the precise measurement of endothelin receptor A levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and accuracy, this kit provides reliable and reproducible results, making it a valuable tool for various research purposes.Endothelin receptor A is a key component in the regulation of vascular tone and blood pressure, making it a crucial target for studying conditions related to cardiovascular health. Dysregulation of this receptor has been linked to conditions such as hypertension, heart failure, and pulmonary arterial hypertension, highlighting its importance as a potential biomarker for diagnosing and monitoring these diseases.
Overall, the Human Endothelin Receptor A CLIA Kit offers researchers a dependable and efficient method for investigating the role of endothelin receptor A in various physiological and pathological processes, paving the way for new insights and therapeutic strategies in the field of cardiovascular research.
Product Name:
Human ETRA (Endothelin Receptor A) CLIA Kit
SKU:
HUES00980
Target:
Human ETRA (Endothelin Receptor A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ETRA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ETRA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ETRA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ETRA. You can calculate the concentration of Human ETRA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
101-112
93-105
Average (%)
100
106
98
1:4
Range (%)
98-110
92-104
102-114
Average (%)
104
98
108
1:8
Range (%)
93-104
98-112
94-108
Average (%)
99
106
100
1:16
Range (%)
103-116
87-97
85-95
Average (%)
109
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
98-111
106
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.85
226.87
824.35
89.6
215.44
790.08
Standard deviation
9.53
18.13
70.65
10.8
23.68
65.34
C V (%)
9.74
7.99
8.57
12.05
10.99
8.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ETRA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ETRA in samples. No significant cross-reactivity or interference between Human ETRA and analogues was observed.
