The Endostatin (ES) ELISA Kit is a sophisticated tool designed for the precise quantification of Endostatin levels in human biological samples. Endostatin, a naturally occurring protein, is involved in critical cellular processes, particularly in angiogenesis inhibition and tumor growth suppression. Accurate detection and measurement of Endostatin are essential for understanding its role in cancer progression, angiogenesis regulation, and potential therapeutic interventions.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control protocols, this kit delivers robust performance and reliability, making it an excellent choice for both clinical diagnostics and research investigations. Trust the Endostatin ELISA Kit from Assay Genie for dependable quantification of this important biomarker in your studies.
Product Name:
Human ES (Endostatin) ELISA Kit
SKU:
AEES00049
Target:
Human ES (Endostatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ES. You can calculate the concentration of Human ES in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
95-107
91-104
Average (%)
97
99
97
1:4
Range (%)
97-107
84-98
99-112
Average (%)
102
90
105
1:8
Range (%)
91-99
84-98
101-110
Average (%)
94
92
105
1:16
Range (%)
100-108
99-109
99-111
Average (%)
105
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-94
91
EDTA plasma (n=5)
95-104
98
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.87
4.86
19.52
1.75
4.63
17.73
Standard deviation
0.1
0.23
0.92
0.09
0.21
0.87
C V (%)
5.35
4.73
4.71
5.14
4.54
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ES in samples. No significant cross-reactivity or interference between Human ES and analogues was observed.
