The Human ER (Estrogen Receptor) Beta CLIA Kit is a highly accurate and sensitive assay designed for the measurement of ER Beta levels in human samples such as serum, plasma, and cell culture supernatants. With its high specificity and reliability, this kit provides consistent and reproducible results, making it an ideal tool for a variety of research applications.ER Beta is a key receptor involved in mediating estrogen signaling and plays a crucial role in processes such as cell proliferation, differentiation, and gene expression.
Dysregulation of ER Beta has been linked to various diseases including breast cancer, prostate cancer, and osteoporosis, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human ER Beta CLIA Kit offers researchers a valuable tool for studying the role of ER Beta in various physiological and pathological conditions, with the potential to advance our understanding of estrogen signaling and its implications for human health.
Product Name:
Human ER beta (Estrogen Receptor Beta) CLIA Kit
SKU:
HUES00661
Target:
Human ER beta (Estrogen Receptor Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ERβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ERβ. You can calculate the concentration of Human ERβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
97-110
84-95
Average (%)
104
103
90
1:4
Range (%)
86-101
100-113
90-106
Average (%)
92
106
97
1:8
Range (%)
97-109
92-107
99-113
Average (%)
103
100
106
1:16
Range (%)
96-109
91-107
103-118
Average (%)
101
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
96-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.8
550.74
1973.2
195.81
515.29
1865.39
Standard deviation
16.45
41.86
160.82
17.23
45.04
164.53
C V (%)
8.53
7.6
8.15
8.8
8.74
8.82
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ERβ concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ERβ in samples. No significant cross-reactivity or interference between Human ERβ and analogues was observed.
