Human EPHX1 Recombinant Protein (RPPB1625)
- SKU:
- RPPB1625
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P07099
- Research Area:
- Enzymes
Description
| Product Name: | Human EPHX1 Recombinant Protein |
| Product Code: | RPPB1625 |
| Size: | 10µg |
| Species: | Human |
| Target: | EPHX1 |
| Synonyms: | Epoxide hydrolase 1, microsomal (xenobiotic), MEH, EPHX, EPOX, Epoxide hydratase, Microsomal epoxide hydrolase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The EPHX1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MASMTGGQQM GRGSHMRDKE ETLPLEDGWW GPGTRSAARE DDSIRPFKVE TSDEEIHDLH QRIDKFRFTP PLEDSCFHYG FNSNYLKKVI SYWRNEFDWK KQVEILNRYP HFKTKIEGLD IHFIHVKPPQ LPAGHTPKPL LMVHGWPGSF YEFYKIIPLL TDPKNHGLSD EHVFEVICPS IPGYGFSEAS SKKGFNSVAT ARIFYKLMLR LGFQEFYIQG GDWGSLICTN MAQLVPSHVK GLHLNMALVL SNFSTLTLLL GQRFGRFLGL TERDVELLYP VKEKVFYSLM RESGYMHIQC TKPDTVGSAL NDSPVGLAAY ILEKFSTWTN TEFRYLEDGG LERKFSLDDL LTNVMLYWTT GTIISSQRFY KENLGQGWMT QKHERMKVYV PTGFSAFPFE LLHTPEKWVR FKYPKLISYS YMVRGGHFAA FEEPELLAQD IRKFLSVLER Q |
Epoxide Hydrolase 1 Microsomal (EPHX1) is a vital biotransformation enzyme which transfers epoxides from the degradation of aromatic compounds to trans-dihydrodiols that can be conjugated and excreted from the body. Epoxide hydrolase plays a role in both activation and detoxification of epoxides. Mutations in EPHX1 trigger preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity.
EPHX1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 451 amino acids (21-455a.a) and having a molecular mass of 52.2kDa. EPHX1 is fused to a 16 amino acid T7-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Ephx1: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls. Defects in EPHX1 are a cause of familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the peptidase S33 family. |
| UniProt Protein Details: | Protein type:EC 3.3.2.9; Xenobiotic Metabolism - metabolism by cytochrome P450; Membrane protein, integral; Hydrolase Chromosomal Location of Human Ortholog: 1q42.1 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity Biological Process: response to toxin; response to organic cyclic substance; aromatic compound catabolic process Disease: Hypercholanemia, Familial; Preeclampsia/eclampsia 1 |
| NCBI Summary: | Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008] |
| UniProt Code: | P07099 |
| NCBI GenInfo Identifier: | 123926 |
| NCBI Gene ID: | 2052 |
| NCBI Accession: | P07099.1 |
| UniProt Secondary Accession: | P07099,Q5VTJ6, Q9NP75, Q9NPE7, Q9NQU6, Q9NQU7, Q9NQU8 Q9NQU9, Q9NQV0, Q9NQV1, Q9NQV2, B2R8N0, |
| UniProt Related Accession: | P07099 |
| Molecular Weight: | 455 |
| NCBI Full Name: | Epoxide hydrolase 1 |
| NCBI Synonym Full Names: | epoxide hydrolase 1, microsomal (xenobiotic) |
| NCBI Official Symbol: | EPHX1�� |
| NCBI Official Synonym Symbols: | MEH; EPHX; EPOX; HYL1�� |
| NCBI Protein Information: | epoxide hydrolase 1; epoxide hydratase |
| UniProt Protein Name: | Epoxide hydrolase 1 |
| UniProt Synonym Protein Names: | Epoxide hydratase; Microsomal epoxide hydrolase |
| UniProt Gene Name: | EPHX1�� |
| UniProt Entry Name: | HYEP_HUMAN |
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