B61,ECKLG,EFL1,EPLG1,LERK1,TNFAIP4,EPH-related receptor tyrosine kinase ligand 1,Tumor necrosis factor alpha-induced protein 4,Immediate early response protein B61
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
The Human Ephrin-A1 (EFNA1) ELISA Kit is specifically designed for the precise measurement of Ephrin-A1 levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results, making it suitable for various research purposes.Ephrin-A1 is a vital protein involved in cell-to-cell communication and plays a crucial role in processes such as cell migration, adhesion, and angiogenesis.
Dysregulation of Ephrin-A1 has been linked to various diseases, including cancer, inflammatory disorders, and neurological conditions, highlighting its importance as a biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Human Ephrin-A1 (EFNA1) ELISA Kit is a valuable tool for researchers looking to investigate the role of Ephrin-A1 in health and disease, offering reliable and robust data for their studies.
Product Name:
Human Ephrin A1 (EFNA1) ELISA Kit
Product Code:
HUDL00914
Size:
96 Assays
Target:
EFNA1
Synonyms:
B61, ECKLG, EFL1, EPLG1, LERK1, TNFAIP4, EPH-related receptor tyrosine kinase ligand 1, Tumor necrosis factor alpha-induced protein 4, Immediate early response protein B61
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
