The Human EP2 (Prostaglandin E Receptor 2) CLIA Kit is a reliable and accurate tool for detecting EP2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.EP2, a prostaglandin E receptor, plays a crucial role in mediating the effects of prostaglandin E2, a key signaling molecule involved in inflammation, pain, and fever. Dysregulation of EP2 signaling has been linked to various diseases such as cancer, inflammation, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker.
With its advanced technology and precise measurements, the Human EP2 CLIA Kit is an invaluable tool for researchers studying EP2 signaling pathways, exploring its role in disease pathogenesis, and developing novel therapeutic strategies. Trust in the accuracy and reliability of this kit to advance your research in the field of prostaglandin E signaling.
Product Name:
Human EP2 (Prostaglandin E Receptor 2 ) CLIA Kit
SKU:
HUES01017
Target:
Human EP2 (Prostaglandin E Receptor 2 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EP2. You can calculate the concentration of Human EP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
90-101
88-103
Average (%)
94
95
95
1:4
Range (%)
97-107
86-99
87-100
Average (%)
102
91
95
1:8
Range (%)
88-103
91-104
92-109
Average (%)
94
98
99
1:16
Range (%)
100-114
94-109
94-110
Average (%)
106
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
101
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
98-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.1
142.02
408.96
52.52
142.12
384.12
Standard deviation
5.57
10.37
39.71
5.06
10.7
27.77
C V (%)
10.11
7.3
9.71
9.63
7.53
7.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EP2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EP2 in samples. No significant cross-reactivity or interference between Human EP2 and analogues was observed.
