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Human Enolase, Non Neuronal (NNE) ELISA Kit

SKU:
HUDL02051
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06733
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ENO1,ENO1L1,MPB1,PPH,Alpha Enolase,Enolase 1,Phosphopyruvate hydratase,Plasminogen-binding protein,2-phospho-D-glycerate hydro-lyase,C-myc promoter-binding
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Enolase, Non Neuronal (NNE) ELISA Kit

The Human ENO2 (Enolase 2) ELISA Kit is a reliable and accurate tool for the quantification of non-neuronal enolase levels in human samples. With a high level of sensitivity and specificity, this kit delivers consistent and reproducible results, making it an excellent choice for various research applications.Enolase 2 is a key enzyme involved in glycolysis and acts as a molecular switch to regulate cell metabolism. Dysregulation of enolase 2 has been associated with various diseases, including cancer, autoimmune disorders, and metabolic syndromes.

Therefore, measuring enolase 2 levels can provide valuable insights into disease progression and potential therapeutic avenues.Overall, the Human ENO2 ELISA Kit offers a powerful tool for researchers to study the role of non-neuronal enolase in health and disease, leading to a better understanding of its functions and potential clinical applications.

Product Name:Human Enolase, Non Neuronal (NNE) ELISA Kit
Product Code:HUDL02051
Size:96 Assays
Target:NNE
Synonyms:ENO1, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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