Human ENO1 Recombinant Protein (RPPB1612)
- SKU:
- RPPB1612
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P06733
- Research Area:
- Enzymes
Description
| Product Name: | Human ENO1 Recombinant Protein |
| Product Code: | RPPB1612 |
| Size: | 25µg |
| Species: | Human |
| Target: | ENO1 |
| Synonyms: | NNE, PPH, MPB1, MBP-1, ENO1L1, ENO1, Alpha-Enolase, Enolase-Alpha, 2-phospho-D-glycerate hydro-lyase, Non-neural enolase, Enolase 1, MPB-1, Phosphopyruvate hydratase, C-myc promoter-binding protein, Plasminogen-binding protein, MBPB1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The ENO1 protein solution (1mg/ml) is formulated in 20mM Tris-HCl pH-7.5 1mM MgSO4 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK |
| Biological Activity: | Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37�C. |
ENO1 is a homodimeric soluble protein that encodes a smaller monomeric structural lens protein, tau-crystallin. ENO1 is a glycolytic enzyme expressed in mainly all tissues. ENO1 isoenzyme full length protein is found in the cytoplasm. The shorter protein is formed from another translation start that is restricted to the nucleus, and binds to a component in the c-myc promoter. ENO1 is involved in anaerobic metabolism under hypoxic conditions and plays a role as a cell surface plasminogen receptor during tissue invasion. Irregular expression of Enolase-1 is linked with tumor progression in several cases of breast and lung cancer. Enolase-1 is as an auto antigen associated with Hashimoto's encephalopathy and severe asthma. ENO1 is the target protein of serum anti-endothelial antibody in Behcet's disease.
The ENO1 Human Recombinant protein is a single, non-glycosilated polypeptide chain produced in E. coli, having a molecular weight of 47.1kDa and containing 434 amino acids (1-434 a.a.).
| UniProt Protein Function: | ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase Chromosomal Location of Human Ortholog: 1p36.2 Cellular Component: cytoplasm; cytosol; extracellular space; M band; membrane; nucleus; phosphopyruvate hydratase complex; plasma membrane Molecular Function:DNA binding; GTPase binding; magnesium ion binding; phosphopyruvate hydratase activity; protein binding; transcription corepressor activity; transcription factor activity Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to virus; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| UniProt Code: | P06733 |
| NCBI GenInfo Identifier: | 119339 |
| NCBI Gene ID: | 2023 |
| NCBI Accession: | P06733.2 |
| UniProt Secondary Accession: | P06733,P22712, Q16704, Q4TUS4, Q53FT9, Q53HR3, Q658M5 Q6GMP2, Q71V37, Q7Z3V6, Q8WU71, B2RD59, |
| UniProt Related Accession: | P06733 |
| Molecular Weight: | 36,928 Da |
| NCBI Full Name: | Alpha-enolase |
| NCBI Synonym Full Names: | enolase 1, (alpha) |
| NCBI Official Symbol: | ENO1�� |
| NCBI Official Synonym Symbols: | NNE; PPH; MPB1; ENO1L1; HEL-S-17�� |
| NCBI Protein Information: | alpha-enolase; c-myc promoter-binding protein-1 |
| UniProt Protein Name: | Alpha-enolase |
| UniProt Synonym Protein Names: | 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein |
| UniProt Gene Name: | ENO1�� |
| UniProt Entry Name: | ENOA_HUMAN |
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