Human ENA 78 Recombinant Protein (RPPB1118)
- SKU:
- RPPB1118
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P42830
- Research Area:
- Chemokines
Description
| Product Name: | Human ENA 78 Recombinant Protein |
| Product Code: | RPPB1118 |
| Size: | 20µg |
| Species: | Human |
| Target: | ENA 78 |
| Synonyms: | Small inducible cytokine B5, CXCL5, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, ENA-78(1-78), chemokine (C-X-C motif) ligand 5, SCYB5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The CXCL5 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized ENA78 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL5 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg |
| Biological Activity: | The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 100ng/mL (corresponding to a Specific Activity of 10,000IU/mg)� of recombinant human ENA-78. |
Chemokine (C-X-C motif) ligand 5 (CXCL5) is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II IFN. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling.
Epithelial Neutrophil-Activating Protein 78 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. The CXCL5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: extracellular space; extracellular region Molecular Function:chemokine activity; CXCR chemokine receptor binding Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; chemotaxis; inflammatory response; signal transduction |
| NCBI Summary: | This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013] |
| UniProt Code: | P42830 |
| NCBI GenInfo Identifier: | 1169525 |
| NCBI Gene ID: | 6374 |
| NCBI Accession: | P42830.1 |
| UniProt Secondary Accession: | P42830,Q96QE1, |
| UniProt Related Accession: | P42830 |
| Molecular Weight: | 11,972 Da |
| NCBI Full Name: | C-X-C motif chemokine 5 |
| NCBI Synonym Full Names: | chemokine (C-X-C motif) ligand 5 |
| NCBI Official Symbol: | CXCL5�� |
| NCBI Official Synonym Symbols: | SCYB5; ENA-78�� |
| NCBI Protein Information: | C-X-C motif chemokine 5; neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; epithelial-derived neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) |
| UniProt Protein Name: | C-X-C motif chemokine 5 |
| UniProt Synonym Protein Names: | ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5Cleaved into the following 2 chains:ENA-78(8-78); ENA-78(9-78) |
| Protein Family: | C-X-C motif chemokine |
| UniProt Gene Name: | CXCL5�� |
| UniProt Entry Name: | CXCL5_HUMAN |
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