Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
The Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit is expertly crafted for the precise and quantitative assessment of EMMPRIN/CD147 levels in a variety of human biological samples. EMMPRIN, also known as CD147, holds a critical role in diverse cellular activities, particularly in modulating extracellular matrix remodeling and facilitating the expression of matrix metalloproteinases. By accurately measuring EMMPRIN/CD147 levels, researchers can gain valuable insights into its involvement in various physiological and pathological processes, including cancer progression, immune responses, and tissue homeostasis.
Our ELISA kit provides exceptional sensitivity and specificity, ensuring the generation of consistent and reliable results crucial for unraveling the multifaceted functions of EMMPRIN/CD147. Crafted under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for cutting-edge research applications. Choose Assay Genie's EMMPRIN/CD147 ELISA Kit for accurate and dependable quantification of this pivotal biomarker in your research endeavors.
Product Name:
Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
SKU:
AEES00159
Target:
Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EMMPRIN/CD147. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EMMPRIN/CD147 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EMMPRIN/CD147, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EMMPRIN/CD147. You can calculate the concentration of Human EMMPRIN/CD147 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-107
92-100
101-108
Average (%)
103
95
105
1:4
Range (%)
85-96
89-95
85-98
Average (%)
91
92
92
1:8
Range (%)
101-109
95-104
88-101
Average (%)
103
98
94
1:16
Range (%)
91-102
89-100
101-109
Average (%)
96
93
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
94-105
98
Cell culture media (n=5)
100-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.18
4.55
0.42
1.23
4.48
Standard deviation
0.03
0.06
0.24
0.02
0.06
0.19
C V (%)
5.56
4.81
5.3
5.28
4.95
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EMMPRIN/CD147 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EMMPRIN/CD147 in samples. No significant cross-reactivity or interference between Human EMMPRIN/CD147 and analogues was observed.
