Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit
The Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit is a reliable and sensitive assay designed for the accurate detection of NBC4 levels in human serum, plasma, and cell culture supernatants. NBC4 is a key protein involved in regulating sodium and bicarbonate ion transport in cells, playing a crucial role in pH homeostasis and acid-base balance.This ELISA kit offers high specificity and reproducibility, making it an ideal tool for research applications in the fields of physiology, neuroscience, and nephrology. With its ability to provide precise measurements of NBC4 levels, researchers can gain valuable insights into the function and regulation of this important transporter protein.
By studying NBC4 levels, researchers can further understand its involvement in various diseases such as hypertension, metabolic disorders, and kidney diseases. With its ability to accurately quantify NBC4 levels in biological samples, this ELISA kit is a valuable tool for advancing scientific research and drug development efforts related to ion transport and pH regulation mechanisms.
Product Name:
Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit
Product Code:
HUDL01990
Size:
96 Assays
Target:
NBC4
Synonyms:
SLC4A5, NBCe2, Solute Carrier Family 4 Member 5,Sodium Bicarbonate Cotransporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
