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Human Elastase 1, Pancreatic (ELA1) ELISA Kit

SKU:
HUDL00928
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UNI1
Sensitivity:
23.6pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
PE,CELA1,Chymotrypsin-Like Elastase Family,Member 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Elastase 1, Pancreatic (ELA1) ELISA Kit

The Human Elastase-1 (Pancreatic ELA1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of elastase-1 levels in human serum, plasma, and cell culture supernatants. Elastase-1, also known as pancreatic elastase, is an important enzyme involved in the digestion of proteins and is often used as a marker for pancreatic function.This ELISA kit offers reliable and reproducible results, making it an essential tool for research in various fields such as gastroenterology, pancreatology, and inflammation.

Monitoring elastase-1 levels can provide valuable insights into pancreatic health and disease, as well as potential applications in drug development and therapeutic interventions.Overall, the Human Elastase-1 (Pancreatic ELA1) ELISA Kit is a versatile and reliable tool for researchers and healthcare professionals looking to investigate the role of elastase-1 in health and disease.

Product Name:Human Elastase 1, Pancreatic (ELA1) ELISA Kit
Product Code:HUDL00928
Size:96 Assays
Target:ELA1
Synonyms:PE, CELA1, Chymotrypsin-Like Elastase Family,Member 1
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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