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Human EIF4EBP1 Recombinant Protein (RPPB3433)

SKU:
RPPB3433
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13541
€309
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Description

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Product Name:Human EIF4EBP1 Recombinant Protein
Product Code:RPPB3433
Size:50µg
Species:Human
Target:EIF4EBP1
Synonyms:Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, 4E-BP1, PHAS-I, EIF4EBP1, BP-1, 4EBP1, MGC4316.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The EIF4EBP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI

EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1) belongs to a family of translation repressor proteins. EIF4EBP1 regulates eIF4E (eukaryotic translation initiation factor 4E) activity by preventing its assembly into the eIF4F complex and mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. EIF4EBP1 is phosphorylated in response to various signals including UV irradiation, resulting in its dissociation from eIF4E and activation of mRNA translation. EIF4EBP1 C-terminus has domains which control function and phosphorylation. EIF4EBP1 has a role in progression of breast neoplasms through cell signaling.

EIF4EBP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 138 amino acids (1-118 a.a.) and having a molecular mass of 14.7kDa (molecular weight on SDS-PAGE will appear higher).The EIF4EBP1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.
UniProt Protein Details:

Protein type:Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function:protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development

NCBI Summary:This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Code:Q13541
NCBI GenInfo Identifier:34921508
NCBI Gene ID:1978
NCBI Accession:Q13541.3
UniProt Secondary Accession:Q13541,Q6IBN3, B2R502, D3DSW8,
UniProt Related Accession:Q13541
Molecular Weight:118
NCBI Full Name:Eukaryotic translation initiation factor 4E-binding protein 1
NCBI Synonym Full Names:eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol:EIF4EBP1��
NCBI Official Synonym Symbols:BP-1; 4EBP1; 4E-BP1; PHAS-I��
NCBI Protein Information:eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
UniProt Protein Name:Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names:Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Protein Family:Eukaryotic translation initiation factor 4E-binding protein
UniProt Gene Name:EIF4EBP1��
UniProt Entry Name:4EBP1_HUMAN

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