Human EIF3I Recombinant Protein (RPPB3427)
- SKU:
- RPPB3427
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13347
Description
| Product Name: | Human EIF3I Recombinant Protein |
| Product Code: | RPPB3427 |
| Size: | 10µg |
| Species: | Human |
| Target: | EIF3I |
| Synonyms: | eIF3-beta, eIF3-p36, EIF3S2, PRO2242, TRIP-1, TRIP1, Eukaryotic translation initiationfactor 3 subunit I, eIF3i, TGF-beta receptor-interacting protein 1, eIF-3-beta. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | EIF3I protein solution (0.25mg/ml) 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 40% Glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDADWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG FDFGGNIIMF STDKQMGYQCFVSFFDLRDP SQIDNNEPYM KIPCNDSKIT SAVWGPLGEC IIAGHESGEL NQYSAKSGEVLVNVKEHSRQ INDIQLSRDM TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPNYDHVVLGGGQ EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY SSGGEDGYVR IHYFDPQYFE FEFEAHHHHH H |
Eukaryotic translation initiation factor 3, subunit I (EIF3I) is part of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for numerous steps in the initiation of protein synthesis. The eIF-3 complex links with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP: methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also essential for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Among the diseases associated with EIF3I are clonorchiasis, and tonsillitis.
EIF3I Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 331 amino acids (1-325 a.a) and having a molecular mass of 37.3kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).EIF3I is fused to an 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | eIF3-beta: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: eukaryotic translation initiation factor 3 complex; cytosol Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation |
| UniProt Code: | Q13347 |
| NCBI GenInfo Identifier: | 2494895 |
| NCBI Gene ID: | 8668 |
| NCBI Accession: | Q13347.1 |
| UniProt Related Accession: | Q13347 |
| Molecular Weight: | 325 |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit I |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3, subunit I |
| NCBI Official Symbol: | EIF3I�� |
| NCBI Official Synonym Symbols: | TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta�� |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit I; EIF3I; eIF3 p36; eIF-3-beta; predicted protein of HQ2242; TGFbeta receptor-interacting protein 1; TGF-beta receptor-interacting protein 1; eukaryotic translation initiation factor 3 subunit 2; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit I |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 2; TGF-beta receptor-interacting protein 1; eIF3 p36 |
| UniProt Gene Name: | EIF3I�� |
| UniProt Entry Name: | EIF3I_HUMAN |
