Human EGFR (Epidermal Growth Factor Receptor) CLIA Kit
The Human EGFR (Epidermal Growth Factor Receptor) CLIA Kit is specifically designed for the quantitative detection of EGFR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.EGFR is a key protein involved in cell growth, division, and differentiation, playing a crucial role in cancer development and progression. Its dysregulation has been linked to various cancers, making it a valuable biomarker for studying cancer biology and developing targeted therapies.
With the Human EGFR CLIA Kit, researchers can gain valuable insights into EGFR levels in biological samples, aiding in the understanding of cancer biology and potentially guiding personalized treatment strategies. This kit is a valuable tool for laboratories conducting research in oncology and related fields.
Product Name:
Human EGFR (Epidermal Growth Factor Receptor) CLIA Kit
SKU:
HUES00058
Target:
Human EGFR (Epidermal Growth Factor Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EGFR. You can calculate the concentration of Human EGFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
99-111
92-107
Average (%)
102
105
100
1:4
Range (%)
95-112
87-102
87-99
Average (%)
103
94
92
1:8
Range (%)
99-114
102-116
84-96
Average (%)
105
107
91
1:16
Range (%)
88-102
92-105
96-110
Average (%)
93
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
273.36
667.43
1782.29
293.44
716.88
1928.34
Standard deviation
31.79
74.22
186.96
30.43
85.81
214.43
C V (%)
11.63
11.12
10.49
10.37
11.97
11.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EGFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGFR in samples. No significant cross-reactivity or interference between Human EGFR and analogues was observed.
