Human EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL-3) ELISA Kit
The Human EDIL3 ELISA Kit is specifically designed for the precise measurement of the human EGF-like repeats and discoidin I-like domains protein 3 (EDIL3) levels in various biological samples, including serum, plasma, and cell culture supernatants. This ELISA kit offers unmatched sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.EDIL3 is a critical protein involved in cell adhesion and tissue development, playing a crucial role in processes such as wound healing, inflammation, and angiogenesis. Dysregulation of EDIL3 has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying disease mechanisms and developing novel therapeutic strategies.
With its advanced technology and superior performance, the Human EDIL3 ELISA Kit is an essential tool for researchers seeking to explore the role of EDIL3 in health and disease, providing valuable insights into potential diagnostic and therapeutic interventions. Order now and accelerate your research with confidence and precision.
Product Name:
Human EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL-3) ELISA Kit
Product Code:
HUDL00909
Size:
96 Assays
Target:
EDIL3
Synonyms:
DEL1, Integrin-binding protein DEL1, Developmentally-regulated endothelial cell locus 1 protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
