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Human EGF DIY ELISA Kit

SKU:
KES0190
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
DIY ELISA
Size:
10 Plates
Synonyms:
EGF, HOMG4, URG
€1,259
Frequently bought together:

Description

system_update_altDatasheet

Human EGF DIY ELISA Kit

The Human EGF (Epidermal Growth Factor) ELISA Kit offered by Assay Genie is designed for the accurate detection of EGF levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications. Epidermal Growth Factor is a key protein involved in cell growth, proliferation, and differentiation. It plays a crucial role in wound healing, tissue repair, and skin regeneration, making it an important biomarker for studying various physiological and pathological processes.

This ELISA kit provides researchers with a reliable tool to quantify EGF levels in biological samples, helping to further understand its role in health and disease. With its user-friendly protocol and fast assay time, the Human EGF ELISA Kit is a valuable resource for studying EGF biology and developing potential therapeutic interventions.

Product Name:Human EGF DIY ELISA
Product Code:KES0190
Species:Human
Target:EGF
Synonyms:EGF, HOMG4, URG
Application:ELISA
ELISA Type:DIY ELISA Kit
Size:10 Plates
Shipping:Room temperature
Storage:Stable for up to twelve months from date of receipt at 2-8°C.
Description Usage Quantity
Anti-Human EGF Polyclonal Antibody Capture Antibody 100 µg
Biotinylated Anti-Human EGF Polyclonal Antibody Detection Antibody 50 µg
Human EGF Recombinant Protein Standard 5 µg
Reagent Suggested Formulation
DPBS: 0.008M sodium phosphate, 0.002M potassium phosphate, 0.14M sodium chloride, 0.01M potassium chloride, pH 7.4
96-well ELISA Plate: Clear, flat-bottom, high-binding 96-well plate, 8-wells per strip, 350 µL per well
(ELISA Plates: KESAP003)
Standard and Sample Diluent: The optimal Standard and Sample Diluent will need to be determined for each sample type to obtain optimal recovery and linearity. The appropriate Standard and Sample Diluent will mimic the sample's response to a known quantity of protein standard and will provide linear results when diluted. Often a 1:4 dilution of the sample in Reagent Diluent will provide acceptable recovery and linearity.
Reagent Diluent and Blocking Buffer: 4% BSA in DPBS, 0.2 µm filtered
Wash Buffer: 0.05% Tween®-20 in DPBS
Streptavidin-HRP: Enzymatic reagent to react with biotinylated detection antibody
(Streptavidin-HRP: KESAP002)
Substrate: 3,3',5,5'-tetramethylbenzidine (TMB) Substrate
(ELISA Accessory Pack: KESAP001)
Stop Solution: 0.18 M Sulfuric Acid
(ELISA Accessory Pack: KESAP001)
Plate Sealer: Adhesive film to prevent evaporation

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step Procedure
1. Prepare Capture Antibody in DPBS at desired working concentration.
2. Add 100 µL of Capture Antibody Working Solution to appropriate wells.
3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 12-24 hours.
4. Empty Capture Antibody Working Solution from plate. Blot plate onto paper towels or other absorbent material.
5. Add 100 µL of Blocking Buffer to appropriate wells.
6. Cover plate with Plate Sealer and incubate at room temperature for 1-3 hours.
7. Empty Blocking Buffer from plate. Blot plate onto paper towels or other absorbent material.
8. Prepare Standard and sample as desired with Standard and Sample Diluent.
9. Add 100 µL of Standard or sample to appropriate wells.
Note: Run each Standard or sample in duplicate.
10. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
11. Wash plate FOUR times with Wash Buffer.
Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material.
12. Prepare Detection Antibody in Reagent Diluent at desired working concentration.
13. Add 100 µL of Detection Antibody Working Solution to each well.
14. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
15. Wash plate FOUR times with Wash Buffer as described in step 11.
16. Prepare Streptavidin-HRP in Reagent Diluent at desired working concentration.
17. Add 100 µL of Streptavidin-HRP Working Solution to each well.
18. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
19. Wash plate FOUR times with Wash Buffer as described in step 11.
20. Add 100 µL of TMB Substrate Solution to each well.
21. Develop the plate in the dark at room temperature for 30 minutes or as desired.
Note: Do NOT cover plate with Plate Sealer.
22. Stop reaction by adding 100 µL of Stop Solution to each well.
23. Measure absorbance on a plate reader at 450 nm.

The Human EGF DIY ELISA kit from Assay Genie can assay for EGF in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Human EGF DIY ELISA Kit from Assay Genie allows researchers to develop their own ELISA plates for EGF using our unique combination of capture and detection antibodies.

Each Human EGF DIY ELISA Kit contains capture antibody, standard, and detection antibody for development of an EGF ELISA. EGF antibodies in this kit have been determined to function in an ELISA with the EGF standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined and require optimisation for the development of this kit. A working knowledge of ELISA is strongly recommended.

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