The Human EGF (Epidermal Growth Factor) ELISA Kit is specifically designed to quantitatively measure EGF levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable tool for researchers in various fields.Epidermal Growth Factor (EGF) is a key protein that plays a vital role in cell growth, proliferation, and differentiation. It is known to be involved in various physiological processes, such as wound healing, tissue regeneration, and development.
Dysregulation of EGF signaling has been linked to various diseases, including cancer and inflammatory disorders.By using the Human EGF ELISA Kit, researchers can gain valuable insights into EGF levels in biological samples, allowing for a better understanding of its role in health and disease. This kit is essential for studying EGF-related pathways and developing potential therapeutic interventions for EGF-associated conditions.
Product Name:
Human EGF (Epidermal Growth Factor) ELISA Kit
SKU:
HUES01346
Target:
Human EGF (Epidermal Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EGF. You can calculate the concentration of Human EGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
95-112
93-108
Average (%)
96
102
100
1:4
Range (%)
101-114
87-99
98-112
Average (%)
107
92
104
1:8
Range (%)
96-111
84-100
91-107
Average (%)
103
91
99
1:16
Range (%)
96-108
85-100
95-108
Average (%)
101
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
13.2
24.1
114.1
13.0
25.8
123.2
Standard deviation
0.7
1.3
5.9
0.7
1.5
3.8
C V (%)
5.3
5.39
5.17
5.38
5.81
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
