The Human EGF (Epidermal Growth Factor) CLIA Kit is specifically designed for the precise measurement of EGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.EGF is a key growth factor that plays a crucial role in cell growth, proliferation, and differentiation. It is involved in a wide range of physiological processes, including wound healing, tissue regeneration, and cancer development.
The ability to measure EGF levels accurately can provide valuable insights into the progression of various diseases and aid in the development of potential therapeutic interventions.By utilizing the Human EGF CLIA Kit, researchers can gain a deeper understanding of the role of EGF in health and disease, ultimately paving the way for advancements in personalized medicine and targeted therapies.
Product Name:
Human EGF (Epidermal Growth Factor) CLIA Kit
SKU:
HUES00057
Target:
Human EGF (Epidermal Growth Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
2.34 pg/mL
Detection range:
3.91-250 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EGF. You can calculate the concentration of Human EGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
98-112
101-117
Average (%)
92
106
110
1:4
Range (%)
100-113
95-109
90-104
Average (%)
107
102
97
1:8
Range (%)
88-101
84-96
99-114
Average (%)
95
91
107
1:16
Range (%)
96-111
91-103
90-106
Average (%)
102
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
95-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
13.31
23.69
113.46
13.89
24.67
112.58
Standard deviation
1.21
2.08
8.82
1.43
1.81
9.96
C V (%)
9.09
8.78
7.77
10.3
7.34
8.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
