The Human EFNA3 (Ephrin-A3) CLIA Kit is a highly reliable and accurate tool for measuring EFNA3 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it a valuable asset for various research endeavors.EFNA3, also known as Ephrin-A3, is a key protein involved in cell signaling and regulation of cell migration. Its dysregulation has been linked to various diseases, including cancer, neurological disorders, and cardiovascular diseases.
By accurately measuring EFNA3 levels, researchers can gain valuable insights into these conditions and potentially uncover new therapeutic strategies.Overall, the Human EFNA3 CLIA Kit from Assaygenie is a powerful tool for studying the role of EFNA3 in disease pathology and developing targeted treatment approaches. Its ease of use and reliability make it a valuable asset for researchers in the fields of oncology, neurology, and cardiology.
Product Name:
Human EFNA3 (Ephrin A3) CLIA Kit
SKU:
HUES00628
Target:
Human EFNA3 (Ephrin A3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EFNA3. You can calculate the concentration of Human EFNA3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-116
99-112
88-100
Average (%)
110
105
93
1:4
Range (%)
84-97
86-98
96-110
Average (%)
91
92
102
1:8
Range (%)
83-97
98-110
85-97
Average (%)
90
103
92
1:16
Range (%)
103-118
91-106
95-110
Average (%)
109
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-104
99
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.53
214.85
923.34
82.93
233.65
902.75
Standard deviation
11.67
15.13
92.24
8.38
20.0
70.96
C V (%)
12.75
7.04
9.99
10.1
8.56
7.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EFNA3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EFNA3 in samples. No significant cross-reactivity or interference between Human EFNA3 and analogues was observed.
