Human EDN2 Recombinant Protein (RPPB1271)
- SKU:
- RPPB1271
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P20800
- Research Area:
- Hormones
Description
| Product Name: | Human EDN2 Recombinant Protein |
| Product Code: | RPPB1271 |
| Size: | 20µg |
| Species: | Human |
| Target: | EDN2 |
| Synonyms: | ET2, PPET2, Endothelin-2, ET-2, Preproendothelin-2, EDN2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | EDN2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSQAAATLE QPASSSHAQG THLRLRRCSC SSWLDKECVY FCHLDIIWVN TPEQTAPYGL GNPPRRRRRS LPRRCQCSSA RDPACATFCL RRPWTEAGAV PSRKSPADVF QTGKTGATTG ELLQRLRDIS TVKSLFAKRQ QEAMREPRST HSRWRKR |
Endothelin-2 is a Hypoxia-induced Autocrine Survival Factor for Breast Tumor Cells. The synthesis of EDN2 by human kidney carcinoma cells is decreased by EGF. EDN2 is a chemoattractant for macrophages and THP-1 monocytic cells. Chemotaxis towards EDN2 is via the MAPK pathway: p44 and p42 are phosphorylated when THP-1 cells are stimulated with EDN2. Migration to EDN2 is inhibited by hypoxia and by pertussis toxin. EDN2 leads to activation of macrophages. EDN2 shares a similar peptide sequence with chemokines and may signal via a similar receptor and MAPK-mediated pathway. Furthermore, EDN2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia.
EDN2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids (25-178 a.a.) and having a molecular mass of 19.9kDa. EDN2 is fused to a 23 amino acid His-tag at N-terminus.
| UniProt Protein Function: | EDN2: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p34 Cellular Component: extracellular region; extracellular space Molecular Function:endothelin B receptor binding; hormone activity Biological Process: artery smooth muscle contraction; calcium-mediated signaling; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; elevation of cytosolic calcium ion concentration; hormonal regulation of the force of heart contraction; inositol phosphate-mediated signaling; macrophage activation; macrophage chemotaxis; neutrophil chemotaxis; positive regulation of cell proliferation; positive regulation of heart rate; positive regulation of leukocyte chemotaxis; positive regulation of the force of heart contraction by chemical signal; prostaglandin biosynthetic process; regulation of systemic arterial blood pressure by endothelin; vasoconstriction; vein smooth muscle contraction |
| NCBI Summary: | This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| UniProt Code: | P20800 |
| NCBI GenInfo Identifier: | 119615 |
| NCBI Gene ID: | 1907 |
| NCBI Accession: | P20800.2 |
| UniProt Secondary Accession: | P20800,Q5T1R3, |
| UniProt Related Accession: | P20800 |
| Molecular Weight: | 19,960 Da |
| NCBI Full Name: | Endothelin-2 |
| NCBI Synonym Full Names: | endothelin 2 |
| NCBI Official Symbol: | EDN2�� |
| NCBI Official Synonym Symbols: | ET2; ET-2; PPET2�� |
| NCBI Protein Information: | endothelin-2 |
| UniProt Protein Name: | Endothelin-2 |
| UniProt Synonym Protein Names: | Preproendothelin-2; PPET2 |
| Protein Family: | Endothelin |
| UniProt Gene Name: | EDN2�� |
| UniProt Entry Name: | EDN2_HUMAN |
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