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Human ECH1 Recombinant Protein (RPPB1604)

SKU:
RPPB1604
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13011
Research Area:
Enzymes
$371
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Description

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Product Name:Human ECH1 Recombinant Protein
Product Code:RPPB1604
Size:20µg
Species:Human
Target:ECH1
Synonyms:peroxisomal, enoyl Coenzyme A hydratase 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The ECH1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 50mM NaCl, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTGSSAQEAA SGVALGEAPD HSYESLRVTS AQKHVLHVQL NRPNKRNAMN KVFWREMVEC FNKISRDADC RAVVISGAGK MFTAGIDLMD MASDILQPKG DDVARISWYL RDIITRYQET FNVIERCPKP VIAAVHGGCI GGGVDLVTAC DIRYCAQDAF FQVKEVDVGL AADVGTLQRL PKVIGNQSLV NELAFTARKM MADEALGSGL VSRVFPDKEV MLDAALALAA EISSKSPVAV QSTKVNLLYS RDHSVAESLN YVASWNMSML QTQDLVKSVQ ATTENKELKT VTFSKL

ECH1 is a member of the hydratase/isomerase superfamily. ECH1 demonstrates high sequence similarity to enoyl-coenzyme A (CoA) hydratases of more than a few species, mostly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence, localizes to both the peroxisome and the mitochondria. peroxisomal takes part in the auxiliary step of the fatty acid beta-oxidation pathway specifically functioning to catalyze the isomerization of 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-transdienoyl-CoA.

ECH1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (34-328a.a.) and having a molecular mass of 34.4kDa.ECH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.
UniProt Protein Details:

Protein type:Mitochondrial; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane

Molecular Function:protein binding; receptor binding

NCBI Summary:This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
UniProt Code:Q13011
NCBI GenInfo Identifier:82654933
NCBI Gene ID:1891
NCBI Accession:Q13011.2
UniProt Secondary Accession:Q13011,Q8WVX0, Q96EZ9, A8K745,
UniProt Related Accession:Q13011
Molecular Weight:35,816 Da
NCBI Full Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
NCBI Synonym Full Names:enoyl-CoA hydratase 1
NCBI Official Symbol:ECH1��
NCBI Official Synonym Symbols:HPXEL��
NCBI Protein Information:delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Protein Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Gene Name:ECH1��
UniProt Entry Name:ECH1_HUMAN

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