Human ECF/CCL11 (Eosinophil Chemotactic Factor) CLIA Kit
The Human ECF (CCL11) ELISA Kit is specifically designed for the precise measurement of eosinophil chemotactic factor levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an indispensable tool for various research applications.Eosinophil chemotactic factor, also known as CCL11, plays a crucial role in eosinophil recruitment and activation, contributing to inflammation and allergic responses.
Elevated levels of CCL11 have been associated with various inflammatory conditions, including asthma, allergic diseases, and autoimmune disorders, highlighting its significance as a potential biomarker for studying these diseases and developing targeted therapies.Get accurate and insightful data on eosinophil chemotactic factor levels with the Human ECF (CCL11) ELISA Kit, and advance your research in the field of inflammation and allergic diseases.
Product Name:
Human ECF/CCL11 (Eosinophil Chemotactic Factor) CLIA Kit
SKU:
HUES00025
Target:
Human ECF/CCL11 (Eosinophil Chemotactic Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ECF/CCL11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ECF/CCL11. You can calculate the concentration of Human ECF/CCL11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
97-111
95-107
Average (%)
92
102
101
1:4
Range (%)
88-100
98-112
85-99
Average (%)
94
104
92
1:8
Range (%)
83-98
96-111
95-111
Average (%)
90
101
102
1:16
Range (%)
92-107
91-104
96-112
Average (%)
98
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
101
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.27
73.24
193.85
26.79
75.05
186.74
Standard deviation
3.37
5.66
18.8
3.19
8.86
13.07
C V (%)
12.36
7.73
9.7
11.91
11.81
7.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ECF/CCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed.
