| Sequence: | Ala 65-Asn 437 |
| Accession: | Q14209 |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation: | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
| Background: | E2F-2 (viral E2-associated factor 2) is a 50‑55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA‑activating E2F heterodimeric complex with DP-1 or -2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F‑2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65‑105), a DNA‑binding domain (aa 107‑196), a dimerization segment (aa 197‑289), a transactivation region (aa 359‑437), and a pRB binding domain (aa 410‑427). There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349‑437. |
