Human DUT Recombinant Protein (RPPB1602)
- SKU:
- RPPB1602
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P33316
- Research Area:
- Enzymes
Description
| Product Name: | Human DUT Recombinant Protein |
| Product Code: | RPPB1602 |
| Size: | 20µg |
| Species: | Human |
| Target: | DUT |
| Synonyms: | Deoxyuridine 5''-triphosphate nucleotidohydrolase mitochondrial, dUTPase, dUTP pyrophosphatase, Deoxyuridine Triphosphatase, DUT, FLJ20622. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DUT solution (1mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN |
Deoxyuridine Triphosphatase (DUT) is a ubiquitous enzyme that functions in nucleotide metabolism. Deoxyuridine Triphosphatase, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is imperative for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive integration of uracil into DNA can eventually lead to cell death. This suggests that DUT is crucial for cell viability, further implying that it is a prospective target for anticancer therapy.
DUT Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids (70-252 a.a.) and having a molecular mass of 21.6kDa. The DUT is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.1.23; DNA replication; Hydrolase; Motility/polarity/chemotaxis; Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator; Mitochondrial Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: nucleoplasm; mitochondrion; nucleus Molecular Function:protein binding; magnesium ion binding; dUTP diphosphatase activity Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process |
| NCBI Summary: | This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P33316 |
| NCBI GenInfo Identifier: | 347595814 |
| NCBI Gene ID: | 1854 |
| NCBI Accession: | P33316.4 |
| UniProt Secondary Accession: | P33316,O14785, Q16708, Q16860, Q6FHN1, Q6NSA3, Q96Q81 A8K650, B4DPR5, |
| UniProt Related Accession: | P33316 |
| Molecular Weight: | 17,748 Da |
| NCBI Full Name: | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial |
| NCBI Synonym Full Names: | deoxyuridine triphosphatase |
| NCBI Official Symbol: | DUT�� |
| NCBI Official Synonym Symbols: | dUTPase�� |
| NCBI Protein Information: | deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase |
| UniProt Protein Name: | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial |
| UniProt Synonym Protein Names: | dUTP pyrophosphatase |
| Protein Family: | Deoxyuridine 5'-triphosphate nucleotidohydrolase |
| UniProt Gene Name: | DUT�� |
| UniProt Entry Name: | DUT_HUMAN |
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