Human DUSP10 Recombinant Protein (RPPB1591)
- SKU:
- RPPB1591
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9Y6W6
- Research Area:
- Enzymes
Description
| Product Name: | Human DUSP10 Recombinant Protein |
| Product Code: | RPPB1591 |
| Size: | 20µg |
| Species: | Human |
| Target: | DUSP10 |
| Synonyms: | Dual specificity protein phosphatase 10, dual specificity phosphatase MKP-5, MKP-5, MAP kinase phosphatase 5, Mitogen-activated protein kinase phosphatase 5, serine/threonine specific protein phosphatase, EC 3.1.3.16, EC 3.1.3.48. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DUSP10 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV |
DUSP10 is a member of the protein-tyrosine phosphatase family. DUSPs inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residuesb and negatively regulate members of the MAPK superfamily which is linked with cellular proliferation and differentiation. DUSP10 interacts with MAPK14 and MAPK8. DUSP10 blocks in mammalian cells the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK.
DUSP10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 359 amino acids (149-482) and having a molecular mass of 40.4kDa.DUSP10 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MKP-5: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. Contains 1 rhodanese domain. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16 Chromosomal Location of Human Ortholog: 1q41 Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus Molecular Function:MAP kinase phosphatase activity; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity Biological Process: inactivation of MAPK activity; JNK cascade; negative regulation of JNK activity; protein amino acid dephosphorylation; response to stress |
| NCBI Summary: | Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q9Y6W6 |
| NCBI GenInfo Identifier: | 20138090 |
| NCBI Gene ID: | 11221 |
| NCBI Accession: | Q9Y6W6.1 |
| UniProt Secondary Accession: | Q9Y6W6,Q6GSI4, Q9H9Z5, D3DTB4, |
| UniProt Related Accession: | Q9Y6W6 |
| Molecular Weight: | 16,110 Da |
| NCBI Full Name: | Dual specificity protein phosphatase 10 |
| NCBI Synonym Full Names: | dual specificity phosphatase 10 |
| NCBI Official Symbol: | DUSP10�� |
| NCBI Official Synonym Symbols: | MKP5; MKP-5�� |
| NCBI Protein Information: | dual specificity protein phosphatase 10 |
| UniProt Protein Name: | Dual specificity protein phosphatase 10 |
| UniProt Synonym Protein Names: | Mitogen-activated protein kinase phosphatase 5; MAP kinase phosphatase 5; MKP-5 |
| Protein Family: | Dual specificity protein phosphatase |
| UniProt Gene Name: | DUSP10�� |
| UniProt Entry Name: | DUS10_HUMAN |
