Human DSTN Recombinant Protein (RPPB3377)
- SKU:
- RPPB3377
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P60981
Description
| Product Name: | Human DSTN Recombinant Protein |
| Product Code: | RPPB3377 |
| Size: | 20µg |
| Species: | Human |
| Target: | DSTN |
| Synonyms: | Destrin (actin depolymerizing factor), ACTDP, ADF, bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP)), destrin, DSN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DSTN solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH |
Actin depolymerizing factor (Destrin/DSTN) belongs to the ADF/Cofilin/destrin superfamily which has the ability to swiftly depolymerize F-Actin in a stoichiometric mode. The ADF family of proteins is responsible for enhancing the turnover rate of actin in vivo. Destrin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. DSTN functions in a pH-independent manner. DSTN is found in a variety of epithelial and endothelial cells, however it is virtually nonexistent in adult mouse heart and skeletal muscle cells. Destrin shares a 71% sequence homology with Cofilin, however the 2 proteins vary in their interaction with Actin.
DSTN Human Recombinant produced in E. coli is a single polypeptide chain containing 173 amino acids (1-165) and having a molecular mass of 19.5 kDa.DSTN is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Destrin: an actin-depolymerizing cytoskeletal protein that belongs to the ADF actin-binding protein family. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Present in many vertebrate tissues and particularly abundant in neuronal cells. It binds F-actin in a pH-dependent manner which affects it's severing/depolymerizing activities. It also binds G-actin at pH 8.0 thereby sequestering monomers and preventing polymerization. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 20p12.1 Cellular Component: actin cytoskeleton Molecular Function:protein binding Biological Process: actin polymerization and/or depolymerization |
| NCBI Summary: | The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P60981 |
| NCBI GenInfo Identifier: | 46577586 |
| NCBI Gene ID: | 11034 |
| NCBI Accession: | P60981.3 |
| UniProt Secondary Accession: | P60981,P18282, Q5W166, Q6IAW2, B2R6N2, B4DYA6, |
| UniProt Related Accession: | P60981 |
| Molecular Weight: | 16,621 Da |
| NCBI Full Name: | Destrin |
| NCBI Synonym Full Names: | destrin, actin depolymerizing factor |
| NCBI Official Symbol: | DSTN�� |
| NCBI Official Synonym Symbols: | ADF; ACTDP; HEL32; bA462D18.2�� |
| NCBI Protein Information: | destrin |
| UniProt Protein Name: | Destrin |
| UniProt Synonym Protein Names: | Actin-depolymerizing factor; ADF |
| Protein Family: | Destrin |
| UniProt Gene Name: | DSTN�� |
| UniProt Entry Name: | DEST_HUMAN |
