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Human DSTN Recombinant Protein (RPPB3377)

SKU:
RPPB3377
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P60981
€309
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Description

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Product Name:Human DSTN Recombinant Protein
Product Code:RPPB3377
Size:20µg
Species:Human
Target:DSTN
Synonyms:Destrin (actin depolymerizing factor), ACTDP, ADF, bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP)), destrin, DSN.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DSTN solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH

Actin depolymerizing factor (Destrin/DSTN) belongs to the ADF/Cofilin/destrin superfamily which has the ability to swiftly depolymerize F-Actin in a stoichiometric mode. The ADF family of proteins is responsible for enhancing the turnover rate of actin in vivo. Destrin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. DSTN functions in a pH-independent manner. DSTN is found in a variety of epithelial and endothelial cells, however it is virtually nonexistent in adult mouse heart and skeletal muscle cells. Destrin shares a 71% sequence homology with Cofilin, however the 2 proteins vary in their interaction with Actin.

DSTN Human Recombinant produced in E. coli is a single polypeptide chain containing 173 amino acids (1-165) and having a molecular mass of 19.5 kDa.DSTN is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Destrin: an actin-depolymerizing cytoskeletal protein that belongs to the ADF actin-binding protein family. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Present in many vertebrate tissues and particularly abundant in neuronal cells. It binds F-actin in a pH-dependent manner which affects it's severing/depolymerizing activities. It also binds G-actin at pH 8.0 thereby sequestering monomers and preventing polymerization.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 20p12.1

Cellular Component: actin cytoskeleton

Molecular Function:protein binding

Biological Process: actin polymerization and/or depolymerization

NCBI Summary:The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P60981
NCBI GenInfo Identifier:46577586
NCBI Gene ID:11034
NCBI Accession:P60981.3
UniProt Secondary Accession:P60981,P18282, Q5W166, Q6IAW2, B2R6N2, B4DYA6,
UniProt Related Accession:P60981
Molecular Weight:16,621 Da
NCBI Full Name:Destrin
NCBI Synonym Full Names:destrin, actin depolymerizing factor
NCBI Official Symbol:DSTN��
NCBI Official Synonym Symbols:ADF; ACTDP; HEL32; bA462D18.2��
NCBI Protein Information:destrin
UniProt Protein Name:Destrin
UniProt Synonym Protein Names:Actin-depolymerizing factor; ADF
Protein Family:Destrin
UniProt Gene Name:DSTN��
UniProt Entry Name:DEST_HUMAN

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