Based on the information found on the provided URL (www.assaygenie.com/human-ds-dermatan-sulfate-elisa-kit-hues02713/), we have the Human DS (Dermatan Sulfate) ELISA Kit. This kit is specifically designed for the precise measurement of dermatan sulfate levels in human samples such as serum, plasma, and cell culture supernatants.With its superior sensitivity and specificity, this ELISA kit ensures accurate and reproducible results, making it an excellent choice for a wide range of research applications. Dermatan sulfate is a key component of the extracellular matrix and is involved in various biological processes including cell adhesion, migration, and growth.
Abnormal levels of dermatan sulfate have been linked to conditions such as Ehlers-Danlos syndrome, Marfan syndrome, and other connective tissue disorders. Therefore, this ELISA kit can be a valuable tool for studying these conditions and exploring potential therapeutic targets.Overall, the Human DS (Dermatan Sulfate) ELISA Kit offers researchers a reliable and efficient method for quantifying dermatan sulfate levels in human samples, providing valuable insights into the role of this molecule in health and disease.
Product Name:
Human DS (Dermatan Sulfate) ELISA Kit
SKU:
HUES02713
Target:
Human DS (Dermatan Sulfate)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DS. You can calculate the concentration of Human DS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
90-101
98-113
Average (%)
105
95
104
1:4
Range (%)
93-104
84-96
84-96
Average (%)
98
89
90
1:8
Range (%)
89-102
81-93
82-93
Average (%)
96
88
87
1:16
Range (%)
86-103
83-94
85-100
Average (%)
94
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.88
6.84
1.03
2.8
7.52
Standard deviation
0.06
0.15
0.35
0.05
0.15
0.3
C V (%)
6.06
5.21
5.12
4.85
5.36
3.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DS in samples. No significant cross-reactivity or interference between Human DS and analogues was observed.
