The Human DPP4 (Dipeptidyl Peptidase IV) ELISA Kit is expertly crafted for the quantitative assessment of DPP4 levels in diverse human biological samples. DPP4, a key enzyme involved in metabolism and immune regulation, plays a crucial role in several physiological processes, particularly in glucose metabolism and immune modulation. The accurate measurement of DPP4 levels is vital for understanding its implications in metabolic disorders, immune responses, and other pathophysiological conditions.
Our ELISA kit offers outstanding sensitivity and specificity, guaranteeing precise and reproducible results essential for thorough investigations into the diverse functions of DPP4. Manufactured under strict quality control measures, this kit ensures robust performance and user-friendly operation, making it an ideal selection for research applications. Count on Assay Genie's DPP4 ELISA Kit for dependable and accurate quantification of this significant biomarker in your research studies.
Product Name:
Human DPP4 (Dipeptidyl Peptidase Ⅳ) ELISA Kit
SKU:
AEES00157
Target:
Human DPP4 (Dipeptidyl Peptidase Ⅳ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DPP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DPP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DPP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DPP4. You can calculate the concentration of Human DPP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-109
98-105
93-104
Average (%)
102
102
98
1:4
Range (%)
92-101
96-105
97-108
Average (%)
95
102
105
1:8
Range (%)
93-102
101-113
93-100
Average (%)
95
105
97
1:16
Range (%)
89-100
97-107
90-100
Average (%)
92
100
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-96
92
EDTA plasma (n=5)
94-105
101
Cell culture media (n=5)
88-98
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.08
5.11
21.75
2.11
5.75
24.52
Standard deviation
0.12
0.25
1.03
0.13
0.28
1.24
C V (%)
5.76
4.84
4.73
5.94
4.79
5.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DPP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DPP4 in samples. No significant cross-reactivity or interference between Human DPP4 and analogues was observed.
