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Human Doublecortin (DCX) ELISA Kit

SKU:
HUDL00800
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O43602
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
DBCN,DC,LISX,SCLH,XLIS,Doublin,Doublecortex,Lissencephalin-X,Lissencephaly,X-Linked,Neuronal migration protein doublecortin
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Doublecortin (DCX) ELISA Kit

The Human Doublecortin (DCX) ELISA Kit is specially developed to detect doublecortin levels in human serum, plasma, and cell culture supernatants with high precision and accuracy. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications.Doublecortin is a vital protein involved in neuronal migration and differentiation, playing a critical role in brain development and function.

Dysregulation of doublecortin has been linked to neurological disorders such as epilepsy, autism, and schizophrenia, making it a valuable biomarker for studying these conditions and exploring potential treatment options. With the Human Doublecortin (DCX) ELISA Kit, researchers can confidently analyze doublecortin levels in biological samples, furthering our understanding of neurodevelopmental processes and neurological diseases.

Product Name:Human Doublecortin (DCX) ELISA Kit
Product Code:HUDL00800
Size:96 Assays
Target:DCX
Synonyms:DBCN, DC, LISX, SCLH, XLIS, Doublin, Doublecortex, Lissencephalin-X, Lissencephaly,X-Linked, Neuronal migration protein doublecortin
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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