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Human Dopa Decarboxylase Recombinant Protein (RPPB1583)

SKU:
RPPB1583
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P20711
Research Area:
Enzymes
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Description

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Product Name:Human Dopa Decarboxylase Recombinant Protein
Product Code:RPPB1583
Size:20µg
Species:Human
Target:Dopa Decarboxylase
Synonyms:DDC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The Dopa decarboxylase protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8, 2mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH TRSMNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE

Dopa decarboxylase is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is involved in 2 metabolic pathways, synthesizing 2 significant neurotransmitters�the take part in numerous clinical disorders, including Parkinson�s disease. Dopa decarboxylase is located in different areas of the brain and is mostly found in basal ganglia. Dopa decarboxylase catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopa, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in Dopa decarboxylase leads to aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that causes combined serotonin and catecholamine deficiency.

Dopa decarboxylase human recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids (1-480 a.a.) and having a molecular mass of 56.4 kDa. The Dopa decarboxylase is fused to a 23 amino acid His Tag at N-terminus and purified by conventional chromatpgraphy.

UniProt Protein Function:DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - tryptophan; EC 4.1.1.28; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Nuclear receptor co-regulator; Amino Acid Metabolism - phenylalanine; Lyase

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: synaptic vesicle; cell soma; axon; cytosol

Molecular Function:aromatic-L-amino-acid decarboxylase activity; protein domain specific binding; amino acid binding; protein binding; enzyme binding; pyridoxal phosphate binding

Biological Process: circadian rhythm; amino acid metabolic process; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid

Disease: Aromatic L-amino Acid Decarboxylase Deficiency

NCBI Summary:The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]
UniProt Code:P20711
NCBI GenInfo Identifier:311033369
NCBI Gene ID:1644
NCBI Accession:P20711.2
UniProt Secondary Accession:P20711,O88533, P14173,
UniProt Related Accession:P20711
Molecular Weight:480
NCBI Full Name:Aromatic-L-amino-acid decarboxylase
NCBI Synonym Full Names:dopa decarboxylase (aromatic L-amino acid decarboxylase)
NCBI Official Symbol:DDC��
NCBI Official Synonym Symbols:AADC��
NCBI Protein Information:aromatic-L-amino-acid decarboxylase
UniProt Protein Name:Aromatic-L-amino-acid decarboxylase
UniProt Synonym Protein Names:DOPA decarboxylase; DDC
Protein Family:Aromatic-L-amino-acid decarboxylase
UniProt Gene Name:DDC��
UniProt Entry Name:DDC_HUMAN

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