The Human DNA Methyltransferase 3B (DNMT3B) ELISA Kit is a highly reliable and sensitive tool for the precise measurement of DNMT3B levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and reproducibility, making it an ideal choice for a wide range of research applications.DNMT3B is an essential enzyme involved in DNA methylation, a crucial epigenetic mechanism that regulates gene expression and plays a key role in various biological processes. Aberrant DNMT3B activity has been implicated in multiple diseases, including cancer, developmental disorders, and neurological conditions, highlighting its significance as a potential therapeutic target and biomarker.
By utilizing the Human DNA Methyltransferase 3B (DNMT3B) ELISA Kit, researchers can gain valuable insights into the regulation of gene expression, the mechanisms underlying disease progression, and the development of novel treatment strategies. This user-friendly and accurate kit is a valuable asset for any laboratory conducting research in the field of epigenetics and molecular biology.
Product Name:
Human DNA Methyltransferase 3B (DNMT3B) ELISA Kit
Product Code:
HUDL00857
Size:
96 Assays
Target:
DNMT3B
Synonyms:
ICF, M.HsaIIIB, DNA methyltransferase HsaIIIB
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
