Human DNA Damage Response Array (27 targets) (SARB0106)
The Human DNA Damage Response Array (SARB0106) is a powerful tool for studying the intricate mechanisms involved in the cellular response to DNA damage. With the ability to simultaneously measure the expression of 27 key targets related to DNA damage response pathways, this array provides researchers with a comprehensive view of how cells react to genotoxic stress.This array includes genes involved in DNA damage sensing, signaling, repair, and cell cycle regulation, allowing for a detailed analysis of the multifaceted response to DNA damage. By assessing the levels of these target genes in response to various treatments or conditions, researchers can gain valuable insights into the underlying mechanisms of DNA damage response and identify potential therapeutic targets for diseases such as cancer.Utilizing a high-throughput, multiplexed approach, the Human DNA Damage Response Array enables researchers to efficiently screen multiple targets in a single experiment, saving time and resources.
The array is compatible with a variety of sample types, including cell lysates and tissue homogenates, making it a versatile tool for studying DNA damage response in a wide range of biological systems.Overall, the Human DNA Damage Response Array (SARB0106) is an essential resource for researchers investigating the complex network of pathways involved in maintaining genomic stability and protecting cells from the detrimental effects of DNA damage. Its comprehensive coverage of key target genes related to DNA damage response makes it a valuable asset for advancing our understanding of DNA repair mechanisms and developing novel therapeutic strategies for treating DNA damage-related diseases.
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process Process
Storage/Stability:
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex
Human DNA Damage Response Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.
Block membranes
2.
Incubate with Sample
3.
Incubate with Biotinylated Detection Antibody Cocktail
4.
Incubate with HRP-Conjugated Streptavidin
5.
Incubate with Detection Buffers
6.
Image with chemiluminescent imaging system
7.
Perform densitometry and analysis
Kinsella et al.
TUMOURS HAVE A SEX: IMPACT ON THE RESPONSE OF BLADDER CANCER AND LUNG ADENOCARCINOMA TO THERAPY
